Welcome Online reservation NMR Services Spectrometers CP800 NMR600

Instrumentation:

  • CP600   :  Bruker 600 MHz spectrometer with a CPTCI cryoprobe

  • NMR600:  Bruker 600 MHz spectrometer with a TXI probe

  • CP800   :  Bruker 800 MHz spectrometer with a CPQCI cryoprobe

  • All equipped with Bruker Avance III console and Topspin3.2 software

Featuring:

  • High resolution, high sensitivity liquid sample NMR measurements

  • Direct 13C, 31P spectrum acquisition

  • High temperature NMR analysis

  • Protein, DNA, RNA, Polysaccharide NMR analysis

Facility Background

Established in year 2008, the Biomolecular NMR Facility is a core facility in the Department of Chemistry and Biochemistry at University of Maryland, College Park. The facility currently hosts three high field liquid NMR spectrometers and has the capability to run a wide range of liquid NMR experiments. Its user base includes scientists from various fields such as organic chemistry, medicinal chemistry, biochemistry, biophysics, polymer science, material science and bioengineering.

Research:

The facility provides high field NMR instrumentation to support University of Maryland research community through measurements of biomolecules, macromolecules and systems of biological interest. It also provides advanced training opportunities for students and acts as a hub for interdisciplinary collaborations.

Services:

The facility provides fast and reliable high resolution liquid NMR analysis to internal and external users. Samples can be organic, inorganic, polymer, biomolecules... Details are on NMR Services webpage.

Main users:

Dr. David Fushman
(Biophysical and Bioanalytical Chemistry, Theoretical and Computational Chemistry):

Development and application of new experimental and theoretical approaches to study structural properties, dynamics, interactions, and regulation in multi-domain systems; polyubiquitin signaling; structure determination and analysis of proteins, nuclear magnetic resonance (NMR) and relaxation; computational biology; molecular dynamics simulations, physical and mathematical modeling.

Dr. Kwaku Dayie
(Bioorganic Chemistry, Biophysical and Bioanalytical Chemistry, Enzymology, Structural Biology, and Theoretical Chemistry):

Structure, interactions, dynamics, and function of RNA complexes involved in catalysis and gene regulation; chemical biological methods of labeling RNAs; NMR methods development.

Dr. Jinwoo Lee
Biochemistry and Biophysics of Membrane Proteins, Membrane Protein NMR, Protein-Protein Interaction, Protein-Lipid Interaction, Viral Membrane Fusion, Magnetic Particle, Kidney Disease

Dr. Lawrence Sita
(Materials Chemistry, Nanoscience and Supramolecular Chemistry, Organometallic Chemistry and Catalysis, Surface Chemistry, Polymer Science and Chemical Technology):

Synthetic, structural and mechanistic inorganic and organometallic chemistry; molecular and mesoscopic self-assembly processes; chemically modified surface and interfaces, polymer synthesis, development and utilization of low cost catalysts for commodity chemical production.

Dr. Paul Paukstelis

Nucleic acid structure, function, and catalysis. Structural DNA nanotechnology. RNA-protein interactions. Structure based drug design.

BioNMR Facility User Policies (Phase II Research, Limited Occupancy Phase, September 1st 2020)

Due to the high contagiousness of COVID-19 virus and severe health issues it may cause, the BioNMR facility is applying restrictions on the facility access and usage. The facility will open for trained users. All users must obey the rules to prevent virus infection and spread.

Current BioNMR Lab Occupancy Limit: 3 users

  1. User Authorization. The BioNMR Facility will continue to be open 24/7 to TRAINED USERS who are authorized by campus for Phase 1 Research. TRAINED USERS are those who have been trained and certified by the Facility Manager for independent operation of specific equipment.
  2. Screening and Logging. All users must complete the Return to UMD Daily Self-Monitoring for COVID-19 survey before coming to the campus. The survey is sent daily to the university email address.
  3. Face Mask and Gloves. All users must wear a face mask at all times. Gloves must also be worn, whenever practical, while at the BioNMR lab. Users must provide their own face mask and gloves. Extra gloves are available in the lab
  4. Advance Reservation. All users must make 24-hour ADVANCE RESERVATIONS on the Facilities. Walk-ins will not be permitted.
  5. Lab Time Management. Users must complete all their measurements and perform necessary and posted sanitations within the scheduled user period.
  6. Work Individually. All users should plan to do their measurements individually, and not with laboratory partners or staff members. For operations that require the specific assistance of a staff member or partner, please contact the Facility Manager directly for guidance. No walk-in consultation until further notice. You may reach the facility director by email, phone, or by scheduled teleconferencing.
  7. COVID-19 Precaution. All users must not come to campus if living with or caring for someone with COVID 19 or if awaiting a COVID 19 test result.
  8. New User Traning. Trainings are available upon request. Users should review and follow the BioNMR Phase II Training Plan
  9. Sample Submission . The BioNMR Facility will continue to run samples for untrained users, or those who wish these expert services. Please contact the facility manager for sample submission.
  10. Consultation. All consultations with the facility manager will be done remotely, via ZOOM or similar mechanisms. All consultations will be done during regular business hours – Monday-Friday 9 am – 5 PM.
  11. Social Distancing. All users must always abide by the following social distancing restrictions (6-foot minimum separation) and posted laboratory occupancy limits.
  12. Safety Awareness Training. All users are required to complete the COVID-19 campus safety awareness training provided by the university

CP800 Computer upgrade to Windows 10 and TopSpin3.6.2, March 2020

The upgraded TopSpin 3.6.2 is on harddrive C, while the NMR data is located under G:/Bruker/TopSpin3.5pl6/data.

  1. The computer and FTP logins are unchanged for most of the regular/active CP800 NMR users. To infrequent or inactive users, please contact the facility manager for re-creating a login.

  2. TopSpin 3.6.2 interface is only slightly different from TopSpin3.5.6. All previously user defined pulseprograms and related files have been transfered to TopSpin 3.6.2. In case any files were not transferred successfully, you may manually copy from TopSpin 3.5.6 folder on G drive to TopSpin 3.6.2 on C drive.

  3. Remote access is allowed for now due to the campus access restriction and Coronavirus crisis. To make Remote access work, first one needs to activate cisco VPN client software on his/her computer, which can be downloaded from Terpware website.
  4. Please note that Remote Desktop may cause computer screen to lock up. Therefore, one will need to come to the lab to unlock the screen or notify the next user to reboot the computer before the next user can access the computer/TopSpin.