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Customers may choose either booking NMR time and run experiments by themselves, or submitting samples to the facility.
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To rerserve NMR time
1. An new customers, as all new users to the facility, needs to demonstrate proficiency in practical NMR operation, or take a two hour training course provided by the facility.
2. Once authorized, the customer will have his/her own NMR account, and be able to reserve time for NMR analysis. Note that for each spectrometer there is a minimal booking block.
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To submit samples to the facility
1. Sample should be sent to the facility with clearly filled out sample submission form (download). First time sample submission requires completion of the account information form (download) as well, for setting up an NMR account.
2. Sample condition: We accept both solid and liquid samples. Recommended liquid sample condition is the following: 1mM sample in deuterated organic solvent or 7% D2O buffer (water based), sample volume no less than 500 µL for a regular NMR tube or 250 µL for a Shigemi NMR tube. A biocide, such as 0.02% Sodium Azide, is encouraged to be added in aqueous sample to prevent bacteria growth.
3. Upon receiving the sample, we will carry out NMR measurements ASAP.
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Data delivery
Acquired NMR Data will be sent to clients electronically, including processed and raw data. Frequent users may request an FTP account for direct data transfer.
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Service area
Fast sample characterization, sample quality monitoring, stucture determination, interaction study, binding site mapping, drug discover...
1. H-1 1D, with or without water suppression; C-13 1D; Saturation transfer difference experiment (STD); Intermolecular hydrogen bonding detection
2. 2D-HSQC, 2D-TOCSY, 2D-NOESY, and more... suitable for both unlabeled and stable isotope labeled samples.
3. Triple resonance experiments, and 3D NMR experiments, which require sample isotope labeling.
4. Consultations on NMR sample preparation (such as protein expression/purification methods and protein stable isotope labeling methods) and experiment selection.
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Fee Structure(Web link)
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