Biomolecular NMR Facility

Department of Chemistry and Biochemistry

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13C DEPT: To differentiate different types of carbons in a chemical compound

C13DEPT

2D Experiments for Chemical Structural Characterization: Looking for connectivity

2D

Saturation Transfer Difference (STD): To identify biomolecule/ligand interactions

STD

Diffusion Ordered Spectroscopy (DOSY): To resolve different compounds in a mixture based on differing translational diffusion coefficients

DOSY

RNA HSQC experiments: Identifying secondary structure elements

RNA

Run triple resonance experiments to establish residue connectivity in a protein sequence

Sequential

NOESY Experiment: To identify proximate protons in space and in adjacent residues

NOESY

NMR Titration: To quantify a protein/ligand interaction, and more...

titration

NMR Evidence of DNA parallel-strand formation in solution

DNA
Comparison of probe sensitity

Probe sensitity